ABSTRACT
We evaluated the effects of Danggui-Chuanxiong (GX) herb pair with different proportions (1∶0, 3∶2, 1∶1, 2∶3, 0∶1) and preparation methods (water extract W, alcohol extract A, and water-alcohol extracts WA) on vasoactive substances and endothelial cell adhesion molecules in the serum of acute blood stasis in rats. An acute blood stasis model was co-replicated by ice water bath and subcutaneous injection of epinephrine hydrochloride in rats. The expressions of vasoactive substances (arachidonic acid metabolites, coagulation-fibrin system index) and adhesion molecules in the serum were detected by enzyme linked immunosorbent assay method; the Spearman method was used to analyze the correlation of those detection indicators; the partial least squares-discriminant analysis and multi-attribute comprehensive index method were used to comprehensively evaluate the total effect of GX herb pair samples with different proportions and preparation methods on vasoactive substances and adhesion molecules. The experimental scheme was approved by the Animal Experimental Ethics Committee of the First Affiliated Hospital of Henan University of Chinese Medicine. The results showed that GX 1∶1_WA had the strongest effect on the improvement of vasoactive substances and adhesion molecules in the serum of acute blood stasis in rats (the total effect value was 6.96). When extraction method was same, the overall effect of GX 1∶1 had better effect than that of other proportions; when the proportion of GX was same, the total effects of GX_WA and GX_A were better than GX_W. The combination of Danggui and Chuanxiong can significantly improve the expressions of vasoactive substances and adhesion molecules in the serum of blood stasis in rats. But the action strength of GX herb pairs was different when the proportions and preparations of GX herb pair were different. These findings provide a basis for clinical rational application of GX herb pair, and lay the foundation for in-depth research on GX herb pair for treatment of blood stasis related diseases.
ABSTRACT
Objective To provide references for clinical rational utilization of TCM injections for activating blood circulation to dissipate blood stasis. Methods Retrospective analysis was used to sort and analyze in terms of species, consumption sum, DDDs and DDDc of TCM injections for activating blood circulation to dissipate blood stasis in Lanzhou Second People's Hospital (here in after referred to as our hospital) during 2014‐2016. Results The consumption sum of TCM injections for activating blood circulation to dissipate blood stasis was relatively high in the consumption sum of TCM injections (over 30％), and the type of TCM injections for activating blood circulation to dissipate blood stasis kept stable (up to 9‐11) in our hospital during 2014‐2016. The annual growth rate of consumption sum in 2016 (-5.42％) was significantly lower than it in 2015 (11.63％). Danshen Injection featured high selectivity and economical in the clinical application (DDDs: 12 600 in 2015, 6600 in 2016; DDDc: 32.70 in 2015, 28.46 in 2016; sorting ratio: 5.00 in 2015, 3.00 in 2016). Conclusion The clinical application of TCM injections for activating blood circulation to dissipate blood stasis tends to be rational in our hospital. Danshen Injection features high selectivity and economical in the clinical application. Danhong Injection features high selectivity but the poor economical.
ABSTRACT
OBJECTIVE:To study the anti-inflammatory effect of Fukang granules (FK,prepared from traditional Chinese herbs,such as Radix Astragali,Radix Codonopsis,Angelica sinensis,etc.). METHODS:The anti-inflammatory action of FK was studied by carrying out rat paw edema test,mouse auricular swelling test,rat chronic pelvic inflammation test and rabbit salpingitis test.RESULTS:FK could lessen rat paw edema,mouse auricular swelling and rat pelvic inflammation and rabbit salpingitis,reduce blood viscosity and blood reduction viscosity and the coefficients of cell aggregation and thrombosis. CONCLUSION:FK has anti-inflammatory effect and the action of activating blood circulation to dissipate blood stasis.